Primary Stimulation and Measurement of Antibody Production to Sheep Red Blood Cells in Vitro
نویسندگان
چکیده
A method for the primary stimulation and measurement of antibody production to sheep red blood cells in vitro has been described. In this system when mouse spleen cells are incubated with sheep erythrocytes large complement-dependent plaques are formed. The number and size of plaques increases from day 1 to day 3 of incubation with an average of 4.4 plaque areas per 1 x 10(6) cells plated at day 3. There is a linear relationship between the number of spleen cells plated and the number of plaques formed. Plaque formation is inhibited by colchicine, actinomycin D, and rabbit anti-mouse globulin. This system offers a possible means for the direct in vitro measurement of the number of cells in a population susceptible to antigenic stimulation by sheep erythrocytes.
منابع مشابه
Haussknechtia Elymatica: A Plant with Immunomodulatory Effects
Background: Plant extracts have been widely investigated for possible immunomodu-latory properties. Objective: To study the immunomodulatory functions of the metha-nol extract of Haussknechtia elymatica (Apioideae), an herb native to south-western Iran. Methods: Delayed type hypersensitivity (DTH) skin test and measurement of an-tibody titer after immunization with Sheep-RBC was performed. [3H]...
متن کاملThe role of platelet microparticles in the production of antibodies from B lymphocytes against HLA-DR antigen in vitro
Background: Platelets can activate B cells and stimulate them for the production of antibodies. Since platelet microparticles (PMPs) originate from platelets, they may have the same virtue. In the present study, the effect of PMPs was investigated on the production of human leukocyte antigen (HLA)-specific antibody from B cells in vitro. Materials and Methods: In this experimental study, HLA-D...
متن کاملDownregulation of IL-12 Production in Healthy Non-Responder Neonates to Recombinant Hepatitis B Vaccine
A proportion of healthy neonates and adults fail to develop a protective antibody response to recombinant hepatitis B (HB) vaccine. Unresponsiveness to vaccination could be attributed to defect in a number of immunological regulatory mechanisms. In this study, IL-12 was quantitated in culture supernatant following in vitro stimulation of peripheral blood mononuclear cells isolated from a group ...
متن کاملEvaluation of Effect of Plasmas Prepared from Packed Red Blood Cells at Different Weeks on Production of Nitric Oxide (NO) and Malondialdehyde (MDA) from Peripheral Blood Mononuclear Cells in Vitro
Introduction: In present study, we evaluated the effect of plasma collected from packed red blood cells (PRBCs) at various weeks after donation on peripheral blood mononuclear cells (PBMCs). Methods: In this experimental study, plasma prepared from twenty PRBCs in the first, second, fourth, and fifth weeks after donation. PBMCs were also isolated from healthy donors and treated with different c...
متن کاملاثر کمبود شدید پروتئین بر روی پاسخهای خاطرهای سیستم ایمنی
ABSTRACT: The effect of protein deficiency on the immune response upon primary and secondary stimulation with 5×108 sheep red blood cells (SRBC) was studied in 4 groups of mice fed by diets containing 4 or 21 percent of protein.Primary and secondary immune response were assessed by quantification of direct and indirect plaque forming cells (PFC) in immune spleens and by titration of serum hema...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
- The Journal of Experimental Medicine
دوره 126 شماره
صفحات -
تاریخ انتشار 1967